Drosophila mauritiana R31 PE genomic DNA library - SRA

SRX688581: Drosophila mauritiana R31 PE genomic DNA library
3 ILLUMINA (Illumina Genome Analyzer IIx) runs: 110M spots, 15.4G bases, 8.4Gb downloads

Design: Genomic DNA was extracted from 15 female flies using a modified Puregene DNA Extraction Protocol. The 15 frozen female flies for each library were lysed by 300 mL cold Cell Lysis Solution (Qiagen, 158906) with pestle homogenization and incubation at room temperature for 15 min, and treated with 4.5 mL RNase A at 37°C for 15 min. Proteins were precipitated out with 100 mL Protein Precipitation Solution (Qiagen, 158910) and centrifugation at 16,000g for 4 min. gDNA in supernatant was purified by DNA Clean and Concentrator –25 Kit (Zymo, D4005) with five volumes of binding buffer to sample. The quality of all gDNA samples was checked by running on an agarose gel, and the concentration was measured with a Qubit Fluorometer (Invitrogen). For the library preparations, 3 mg gDNA was sheared to 300 bp with Covaris, followed with end-repairing, A-tailing, ligating to adaptors, selecting size between 350 and 450 bp with 2% agarose gel, and PCR amplifying for 15 cycles. The concentration of the sample was measured with Qubit Fluorometer (Invitrogen) and the libraries were diluted to a concentration of 10 nM for sequencing.

Submitted by: University of Rochester

Study: Drosophila mauritiana Genome sequencing

PRJNA158675 • SRP012053 • All experiments • All runs

show Abstracthide Abstract

The species of the Drosophila melanogaster subgroup are important models for studying the molecular basis of the origin of species. While many of the species in this subgroup have an existing genome sequence, D. mauritiana does not. D. mauritiana is a widely used taxon in speciation genetics. Our goal is to obtain a high-quality reference sequence for investigators using D. mauritiana for their research.

Sample:

SAMN02999508 • SRS691151 • All experiments • All runs

Organism: Drosophila mauritiana

Library:

Instrument: Illumina Genome Analyzer IIx

Strategy: WGS

Source: GENOMIC

Selection: size fractionation

Layout: PAIRED

Spot descriptor:

1 forward77 reverse

Runs: 3 runs, 110M spots, 15.4G bases, 8.4Gb

Run	# of Spots	# of Bases	Size	Published
SRR1560095	30,818,260	3.3G	1.6Gb	2015-07-22
SRR1560097	35,611,755	5.4G	3Gb	2015-07-22
SRR1560098	43,525,329	6.6G	3.8Gb	2015-07-22

ID:: 962858

SRA

Sequence Read Archive

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